Extracting DNA of nematodes communities from Argentine Pampas agricultural soils

We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems.

Bibliographic Details

Main Authors:	Mondino, Eduardo Ariel, Covacevich, Fernanda, Studdert, Guillermo, Pimentel, João Pedro, Berbara, Ricardo Luiz Louro
Format:	info:ar-repo/semantics/artículo biblioteca
Language:	eng
Published:	2015
Subjects:	Suelos Agrícolas, Nematoda, ADN, Agroecosistemas, Agroecosystems, DNA, Agricultural Soils, Región Pampeana, Cetyltrimethylammonium Bromide, CTAB,
Online Access:	http://hdl.handle.net/20.500.12123/2360 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691 http://dx.doi.org/10.1590/0001-3765201520130110
Tags:	Add Tag No Tags, Be the first to tag this record!