Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex.
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | spa |
| Published: |
Elsevier
2021-03-10
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| Subjects: | Soja, Tallos, Diaporthe, Agrobacterium Tumefaciens, Infecciones Latentes, Soybeans, Stems, Latent Infection, Diaporthe caulivora, |
| Online Access: | http://hdl.handle.net/20.500.12123/10234 https://www.sciencedirect.com/science/article/pii/S0167701221000658 https://doi.org/10.1016/j.mimet.2021.106197 |
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