An in Vitro Biocompatibility Study of Conventional and Resin-modified Glass Ionomer Cements
Purpose: To evaluate the biocompatibility of a glass-ionomer (GIC) and a resin-modified glass-ionomer cement (RM-GIC), cell viability was examined in a model of human gingival fibroblasts using morphological, biochemical, and ionic patterns by means of phase contrast microscopy, lactate dehydrogenase (LDH) release, and quantitative x-ray microanalysis (EPXMA). Materials and Methods: The GIC Ketac-Molar Easymix (3M ESPE) and the RM-GIC Vitrebond (3M ESPE) were compared in human gingival fibroblasts exposed to the cements for 72 h. As controls, fibroblasts cultured with DMEM culture medium (negative control) and with 1% triton × (positive control) were used. Results: Light microscopic findings showed greater morphological alterations in cells exposed to RM-GIC than to GIC. The relative percentage of LDH released from the cells to the supernatant was significantly higher in RMGIC cultures than in the control. Quantitative x-ray microanalysis showed that cultures exposed to RM-GIC were characterized by an increase in intracellular Na and a decrease in intracellular Cl and K. These changes in ion composition were significant compared to control and GIC cultures. Conclusion: The three indicators of cellular biocompatibility after 72 h of exposure showed that RM-GIC led to more marked alterations than GIC in human gingival fibroblasts.
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| Format: | article biblioteca |
| Langue: | eng |
| Publié: |
2013
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| Sujets: | GLASS-IONOMER CEMENTS; BIOCOMPATIBILITY; INTRACELLULAR; ELECTRON PROBE MICROANALYSIS, |
| Accès en ligne: | http://dx.doi.org/10.3290/j.jad.a29588 http://hdl.handle.net/11086/13456 |
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