A high-performance liquid chromatographic method for quantitation of DIMBOA and MBOA in maize plant extract
A high-pressure liquid chromatographic (LC) method for quantitation of DIMBOA [2,4-dihydroxy-7-methoxy-2H-l,4-benzoxacin-3(4H)-one] and its main degradation product MBOA [6-methoxy-2-(3H)-benzoxazolinone] in aqueous extract from corn (Zea mays L.) is described. After recovering DIMBOA and MBOA from the aqueous extract with ethyl acetate, underivatized samples were chromatographed on LiChrosorb SI 100 by using a hexane-ethanol gradient and monitored at 288 nm. A typical LC pattern shows five main peaks, two of them eluting respectively at the same position as the MBOA and DIMBOA standards and enhanced with the addition to the sample of these compounds. Variation coefficients of 1.11% and 1.07% were respectively obtained for the molar responses of MBOA and DIMBOA standards. For the retention times coefficients of variation of 0.011% for MBOA and 0.019% for DIMBOA were determined. The samples recoveries ranged from 104 ± 2% for DIMBOA to 106 ±3% for MBOA. © 1982, American Chemical Society. All rights reserved.
| Main Authors: | , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
1982
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| Online Access: | http://hdl.handle.net/20.500.12792/5179 |
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