Agrobacterium-mediated transformation of cork oak (Quercus suber L.) somatic embryos
A simple method is presented for the unreported genetic transformation of cork oak (Quercus suber L.). Pro-embryo masses (PEMs) were induced on immature zygotic embryos applied to medium supplemented with 2.3 μM 2,4-dichlorophenoxyacetic acid. The established PEMs were inoculated with Agrobacterium tumefaciens LBA4404/p35S GUS INT/pCAMBIA 1301 strain. Transformants were selected on hygromycin (HYG) 94 μM-supplemented medium. Viable embryos constituted 13% of those selected on HYG during 4 months. Expression of β-glucuronidase at 4 months following co-cultivation confirmed transformation in 5.8% embryos selected on HYG. This method forms a basis for genetic transformation of cork oak somatic embryos. © Springer 2005.
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| Main Authors: | , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
2005
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| Online Access: | http://hdl.handle.net/20.500.12792/4042 |
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