Use of enzymatic cDNA amplification as a method of detection of bean yellow mosaic virus
The C-terminal region of bean yellow mosaic virus (BYMV) coat protein gene was selected for the design of oligonucleotide primers. Reverse transcription of viral RNA present in sap of virus-infected plants followed by polymerase chain reaction (RT-PCR) was performed for amplification of a 449 baise pairs fragment. These primers supported BYMV amplification, but did not support amplification on both bean common mosaic virus (BCMV) and potato virus Y (PVY) templates. © 1993 Koninklijke Nederlandse Planteziektenkundige Vereniging.
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| Main Authors: | , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Kluwer Academic Publishers
1993
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| Subjects: | Detection, Bean common mosaic virus, RT-PCR, Potato virus Y, |
| Online Access: | http://hdl.handle.net/20.500.12792/5830 http://hdl.handle.net/10261/295165 |
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