An appraisal of different methods for the detection of the walnut strain of cherry leafroll virus
Three methods were evaluated for the detection of cherry leafroll virus ELISA, dot-blot and reverse transcriptional polymerase chain reaction (RT-PCR). Dot-blot and RT-PCR were carried out in crude plant extracts without any further RNA purification. Dot-blot hybridization using a 32P-labelled DNA probe was as sensitive as previously reported ELISA results for cherry leafroll virus detection. The most sensitive method was RT-PCR, which amplified a specific fragment of 448 bp from the 3' untranslated region of both viral genomic RNAs. RT-PCR was used to detect cherry leafroll virus in infected walnut buds and twigs. © 1992.
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| Main Authors: | , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
1992
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| Subjects: | Detection, Cherry leafroll virus, ELISA, Dot-blot, Polymerase chain reaction, Reverse transcriptional, Walnut, |
| Online Access: | http://hdl.handle.net/20.500.12792/4909 http://hdl.handle.net/10261/294810 |
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