Arabidopsis synaptotagmin 1 is required for the maintenance of plasma membrane integrity and cell viability

Plasma membrane repair in animal cells uses synaptotagmin 7, a Ca 2+-activated membrane fusion protein that mediates delivery of intracellular membranes to wound sites by a mechanism resembling neuronal Ca2+-regulated exocytosis. Here, we show that loss of function of the homologous Arabidopsis thaliana Synaptotagmin 1 protein (SYT1) reduces the viability of cells as a consequence of a decrease in the integrity of the plasma membrane. This reduced integrity is enhanced in the syt1-2 null mutant in conditions of osmotic stress likely caused by a defective plasma membrane repair. Consistent with a role in plasma membrane repair, SYT1 is ubiquitously expressed, is located at the plasma membrane, and shares all domains characteristic of animal synaptotagmins (i.e.;an N terminus-transmembrane domain and a cytoplasmic region containing two C2 domains with phospholipid binding activities). Our analyses support that membrane trafficking mediated by SYT1 is important for plasma membrane integrity and plant fitness. © 2008 American Society of Plant Biologists.

Bibliographic Details

Main Authors:	Schapire, A. L., Voigt, B., Jasik, J., Rosado, A., Lopez-Cobollo, R., Menzel, D., Salinas, J., Mancuso, S., Valpuesta, V., Baluska, F., Botella, M. A.
Format:	journal article biblioteca
Language:	English
Published:	Oxford University Press 2008
Online Access:	http://hdl.handle.net/20.500.12792/4266 http://hdl.handle.net/10261/294603
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