Analysis of functional heterogeneity of porcine memory CD4+ T cells
Previous studies have identified swine helper memory T cells as CD4 +CD8α+SLADR+. We have recently described a new porcine surface antigen (2E3) selectively expressed on CD4+ T cells that allows to divide these cells into naïve (2E3+) and effector/memory (2E3-). However, although the majority of CD4 +2E3- cells are CD8α+SLADR+, a minor proportion do not express SLADR and/or CD8α. Here, we have analyzed the functional capacity of these CD4+2E3- subsets to proliferate to a recall antigen. Both SLADR- and CD8α - cells proliferated in response to lysozyme, but at lower levels compared to the whole population CD4+2E3-. Besides, after activation with PMA plus ionomycin, CD4+2E3-SLADR - T cells produced IFNγ and TNFα, although they did also in lower proportion than the whole CD4+2E3- population. Most of the IFNγ-TNFα+, IFNγ +TNFα+, IFNγ+TNFα- cells were CD8α+ and CD45RA-, while IFNγ-TNFα- cells showed a less differentiate phenotype. © 2004 Elsevier Ltd. All rights reserved.
| Main Authors: | , , , , , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
2005
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| Subjects: | Swine, T Lymphocytes, Cell surface molecules, Cellular activation, Memory T lymphocytes, Naïve T Lymphocytes, Cytokines, |
| Online Access: | http://hdl.handle.net/20.500.12792/5416 http://hdl.handle.net/10261/294004 |
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