Analysis of chemokine receptor CCR7 expression on porcine blood T lymphocytes using a CCL19-Fc fusion protein
The chemokine receptor CCR7 has been a useful marker for the characterization of human and mouse T cell subsets. We have produced the porcine CCR7 ligand CCL19 fused to the human IgG1 Fc fragment, and used it to analyse CCR7 expression in swine. CCL19-Fc bound to and induced the migration of cells expressing porcine CCR7 but not of untransfected cells, corroborating its specificity. On blood lymphocytes, CCL19-Fc labelled the majority of CD4+ T cells expressing the 2E3 marker, associated with a naïve phenotype, whereas the 2E3- cells were mostly negative. Among CD8+ T cells CCL19-Fc labelled two subsets one, CD8βhi CD11alo CD45RA+, perforin-/lo , which produced low amounts of IFN-γ after stimulation, which might correspond to naïve cells; and a second small population of CD8βlo cells which expressed high levels of CD11a, and were mostly CD45RA-, a phenotype which resembles that of human central memory T cells. © 2012 Elsevier Ltd.
Main Authors: | , , , , , , , |
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Format: | journal article biblioteca |
Language: | English |
Published: |
Elsevier
2013
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Subjects: | Swine, Chemokines, CCL19, CCR7, T cell subsets, |
Online Access: | http://hdl.handle.net/20.500.12792/3013 http://hdl.handle.net/10261/293550 |
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