Fast and simultaneous determination of phenolic compounds and caffeine in teas, mate, instant coffee, soft drink and energetic drink by high-performance liquid chromatography using a fused-core column
A fast HPLC method with diode-array absorbance detector and fluorescence detector for the analysis of 19 phenolic acids, flavan-3-ols, flavones, flavonols and caffeine in different types of samples was developed. Using a C18 reverse-phase fused-core column separation of all compounds was achieved in less than 5min with an overall sample-to-sample time of 10min. Evaluation of chromatographic performance revealed excellent reproducibility, resolution, selectivity and peak symmetry. Limits of detection for all analyzed compounds ranged from 0.5 to 211μgL-1, while limits of quantitation ranged between 1.5 and 704μgL-1. The developed method was used for the determination of analytes present in different samples, including teas (black, white, green), mate, coffee, cola soft drink and an energetic drink. Concentration of the analyzed compounds occurring in the samples ranged from 0.4 to 314mgL-1. Caffeine was the analyte found in higher concentrations in all samples. Phytochemical profiles of the samples were consistent with those reported in the literature. © 2010 Elsevier B.V.
| Main Authors: | , , , , , , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
2011
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| Subjects: | Fast separations, Phenolic acids, Flavan-3-ols, Flavones, Flavonols, Caffeine, HPLC, Fused-core, |
| Online Access: | http://hdl.handle.net/20.500.12792/5437 http://hdl.handle.net/10261/293254 |
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