Development of highly polymorphic tetranucleotide microsatellite markers in Austrocedrus chilensis

An enriched genomic library was constructed and 9 novel polymorphic tetranucleotide microsatellite markers developed for Austrocedrus chilensis, the most economically important native conifer in the Andean Patagonian region. Polymorphism was investigated for these markers in 48 individuals from two populations. Numbers of alleles ranged from 3 to 19 and levels of observed heterozygosity among the 9 loci ranged from 0.32 to 0.95. No locus combinations exhibited linkage disequilibrium. These polymorphic markers will be useful tools for the study of demography and gene flow and more in general for population and conservation genetics of this species. © 2008 The Authors.

Bibliographic Details

Main Authors:	Arana, M. V., Buonamici, A., Sebastiani, F., Alía Miranda, Ricardo, Gallo, L. A., Marchelli, P., Moreno, C., Vendramin, G. G.
Format:	artículo biblioteca
Language:	English
Published:	Wiley 2008
Subjects:	Austrocedrus chilensis, Enriched library, Microsatellites,
Online Access:	http://hdl.handle.net/20.500.12792/1297 http://hdl.handle.net/10261/292053
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