Vaccination with recombinant adenovirus expressing peste des petits ruminants virus-F or -H proteins elicits T cell responses to epitopes that arises during PPRV infection

Peste des petits ruminants virus (PPRV) causes an economically important disease that limits productivity in small domestic ruminants and often afects the livestock of the poorest populations in developing countries. Animals that survive PPRV develop strong cellular and humoral responses, which are probably necessary for protection. Vaccination should thus aim at mimicking these natural responses. Immunization strategies against this morbillivirus using recom_x0002_binant adenoviruses expressing PPRV-F or -H proteins can protect PPRV-challenged animals and permit diferentia_x0002_tion of infected from vaccinated animals. Little is known of the T cell repertoire these recombinant vaccines induce. In the present work, we identifed several CD4+ and CD8+ T cell epitopes in sheep infected with PPRV. We also show that recombinant adenovirus vaccination induced T cell responses to the same epitopes, and led to memory T cell diferentiation. T cells primed by these recombinant adenovirus vaccines expanded after PPRV challenge and prob_x0002_ably contributed to protection. These data validate the use of recombinant adenovirus expressing PPRV genes as DIVA strategies to control this highly contagious disease.

Détails bibliographiques

Auteurs principaux:	Rojas Carrasco, José Manuel, Avia, M., Pascual, E., Sevilla Hidalgo, Noemi, Martín, V.
Format:	artículo biblioteca
Langue:	English
Publié:	BioMed Central 2017
Accès en ligne:	http://hdl.handle.net/20.500.12792/3866 http://hdl.handle.net/10261/291549
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