rab3-Peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A2 metabolites
Acrosomal exocytosis triggered with A23187/Ca2+ was enhanced by rab3AL, a synthetic peptide corresponding to the effector domain of the small GTP-binding protein rab3. Exocytosis was further enhanced when spermatozoa were also exposed to dibutyryl-cAMP, but was prevented when H-89, a protein kinase A (PKA) inhibitor, was included. The action of rab3AL was not on, or upstream of, phospholipase A2 (PLA2). Inhibition of exocytosis by the PLA2 inhibitor aristolochic acid was overcome by rab3AL when it was included together with lysophosphatidylcholine; this effect was prevented by H-89. These results suggest a functional coupling between rab3 protein, metabolites generated by PLA2, and cAMP-activated PKA, in the final steps leading to membrane fusion during acrosomal exocytosis.
| Principais autores: | , |
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| Formato: | artículo biblioteca |
| Idioma: | English |
| Publicado em: |
John Wiley & Sons
1996
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| Assuntos: | Exocytosis, Rab3, Phospholipase Az, Lysophosphatidylcholine, CAMP, Spermatozoa, |
| Acesso em linha: | http://hdl.handle.net/20.500.12792/1657 http://hdl.handle.net/10261/291453 |
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