RT-PCR in foot-and-mouth

A RT-PCR assay for the specific detection of RNA sequences from foot-and-mouth disease virus (FMDV) has been developed. The procedure permits also the detection of sequences that correlate with established FMDV serotypes. A computer program that allows selection of genotype-specific primers for RT-PCR amplification was used for the identification of FMDV specific sequences for PCR amplification on RNA replicase (3D) gene regions. Specific, rapid and highly sensitive detection was achieved for a wide collection of RNA samples from FMDV types C, A and O, either purified from tissue culture or extracted from lesions of infected animals. Similarly, serotype-specific primers were designed to amplify the carboxy-terminal end of the VP1 gene of FMDV types either C, A or O. The results of PCR amplification of diffefent FMDV RNAs using type-specific primers are in agreement with the serological typing of the corresponding viruses. A combination of this approach with a simplified sample processing, carried out following direct adsorption of viral suspensions to microtiter plates, provides a rapid, reliable method of viral diagnosis. © 1998 Taylor & Francis Group, LLC.

Bibliographic Details

Main Authors:	Dopazo, J., Núñez, J. I., Blanco Lavilla, Esther, Hernández, T., Sobrino, F.
Format:	artículo biblioteca
Language:	English
Published:	Sage Publications 1998
Online Access:	http://hdl.handle.net/20.500.12792/1136 http://hdl.handle.net/10261/290506
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