In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus

T-cell epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) glycoproteins 4 (GP4), 5 (GP5) and nucleocapsid (N) were predicted using bioinformatics and later tested by IFN-γ ELISPOT in pigs immunized with either a modified live vaccine (MLV) or DNA (open reading frames 4, 5 or 7). For MLV-vaccinated pigs, immunodominant epitopes were found in N but T-epitopes were also found in GP4 and GP5. For DNA-immunized pigs, some peptides were differently recognized. Using a large set of PRRSV sequences it was shown that N contains a conserved epitope and that for GP5, the genotype-I counterparts of previously reported epitopes of genotype-II strains were also immunogenic. © 2009 Elsevier Ltd. All rights reserved.

Bibliographic Details

Main Authors:	Díaz, I., Pujols, J., Ganges, L., Gimeno, M., Darwich, L., Domingo, M., Mateu, E.
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2009
Subjects:	Porcine reproductive and respiratory syndrome virus, T-cell epitopes, Bioinformatics,
Online Access:	http://hdl.handle.net/20.500.12792/1064 http://hdl.handle.net/10261/289743
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