Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation
10 páginas, 7 figuras.
Guardado en:
| Autores principales: | , , , , , , , , , , , |
|---|---|
| Otros Autores: | |
| Formato: | artículo biblioteca |
| Publicado: |
Elsevier
2021
|
| Materias: | Chicory, Anthelmintics, Parasite, Sesquiterpene lactone, |
| Acceso en línea: | http://hdl.handle.net/10261/245609 http://dx.doi.org/10.13039/501100002848 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
dig-igm-es-10261-245609 |
|---|---|
| record_format |
koha |
| spelling |
dig-igm-es-10261-2456092022-05-24T10:37:53Z Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation Valente, A. H. De Roode, M. Ernst, Madeleine Pena-Espinoza, M. Bornancin, L. Bonde, C. Martínez Valladares, María Ramunke, S. Krucken, J. Simonsen, H.T. Thamsborg, S. M. Williams, A. R. University of Copenhagen Danish Council for Independent Research Danish Agricultural Agency Comisión Nacional de Investigación Científica y Tecnológica (Chile) Martínez Valladares, María [0000-0002-3723-1895] Chicory Anthelmintics Parasite Sesquiterpene lactone 10 páginas, 7 figuras. Increasing resistance towards anthelmintic drugs has necessitated the search for alternative treatments for the control of gastrointestinal nematode parasites. Animals fed on chicory (Cichorium intybus L.), a temperate (pasture) crop, have reduced parasite burdens, hence making C. intybus a potentially useful source for novel anthelmintic compounds or a diet-based preventive/therapeutic option. Here, we utilized in vitro bioassays with the parasitic nematode Ascaris suum and molecular networking techniques with five chicory cultivars to identify putative active compounds. Network analysis predicted sesquiterpene lactones (SL) as the most likely group of anthelmintic compounds. Further bioassay-guided fractionation supported these predictions, and isolation of pure compounds demonstrated that the SL 8-deoxylactucin (8-DOL) is the compound most strongly associated with anti-parasitic activity. Furthermore, we showed that 8-DOL acts in a synergistic combination with other SL to exert the anti-parasitic effects. Finally, we established that chicory-derived extracts also showed activity against two ruminant nematodes (Teladorsagia circumcincta and Cooperia oncophora) in in vitro assays. Collectively, our results confirm the anti-parasitic activity of chicory against a range of nematodes, and pave the way for targeted extraction of active compounds or selective breeding of specific cultivars to optimize its future use in human and veterinary medicine. We are very grateful for the guidance and support by Dr. Thomas Ostenfeld Larsen and Christopher Phippen, Technical University of Denmark, Natural product discovery, and the laboratory assistance of Mette Schjelde, University of Copenhagen. This work was funded by the Danish Council for Independent Research (Grant DFF–6111-00394). Fractionation and purification of compounds were further supported by the Green Development and Demonstration Program (GUDP) (Project No. 34009-17-1220). MPE was supported by CONICYT Chile (FONDE-CYT Postdoctorado #3170875 2021-07-09T12:00:02Z 2021-07-09T12:00:02Z 2021 2021-07-09T12:00:02Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.ijpddr.2021.02.002 issn: 2211-3207 International Journal for Parasitology: Drugs and Drug Resistance 15: 105- 114 (2021) http://hdl.handle.net/10261/245609 10.1016/j.ijpddr.2021.02.002 http://dx.doi.org/10.13039/501100002848 Publisher's version http://dx.doi.org/10.1016/j.ijpddr.2021.02.002 Sí open Elsevier |
| institution |
IGM ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-igm-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del IGM España |
| topic |
Chicory Anthelmintics Parasite Sesquiterpene lactone Chicory Anthelmintics Parasite Sesquiterpene lactone |
| spellingShingle |
Chicory Anthelmintics Parasite Sesquiterpene lactone Chicory Anthelmintics Parasite Sesquiterpene lactone Valente, A. H. De Roode, M. Ernst, Madeleine Pena-Espinoza, M. Bornancin, L. Bonde, C. Martínez Valladares, María Ramunke, S. Krucken, J. Simonsen, H.T. Thamsborg, S. M. Williams, A. R. Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation |
| description |
10 páginas, 7 figuras. |
| author2 |
University of Copenhagen |
| author_facet |
University of Copenhagen Valente, A. H. De Roode, M. Ernst, Madeleine Pena-Espinoza, M. Bornancin, L. Bonde, C. Martínez Valladares, María Ramunke, S. Krucken, J. Simonsen, H.T. Thamsborg, S. M. Williams, A. R. |
| format |
artículo |
| topic_facet |
Chicory Anthelmintics Parasite Sesquiterpene lactone |
| author |
Valente, A. H. De Roode, M. Ernst, Madeleine Pena-Espinoza, M. Bornancin, L. Bonde, C. Martínez Valladares, María Ramunke, S. Krucken, J. Simonsen, H.T. Thamsborg, S. M. Williams, A. R. |
| author_sort |
Valente, A. H. |
| title |
Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation |
| title_short |
Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation |
| title_full |
Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation |
| title_fullStr |
Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation |
| title_full_unstemmed |
Identification of compounds responsible for the anthelmintic effects of chicory (Cichorium intybus) by molecular networking and bio-guided fractionation |
| title_sort |
identification of compounds responsible for the anthelmintic effects of chicory (cichorium intybus) by molecular networking and bio-guided fractionation |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10261/245609 http://dx.doi.org/10.13039/501100002848 |
| work_keys_str_mv |
AT valenteah identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT deroodem identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT ernstmadeleine identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT penaespinozam identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT bornancinl identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT bondec identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT martinezvalladaresmaria identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT ramunkes identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT kruckenj identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT simonsenht identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT thamsborgsm identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation AT williamsar identificationofcompoundsresponsiblefortheanthelminticeffectsofchicorycichoriumintybusbymolecularnetworkingandbioguidedfractionation |
| _version_ |
1777664092587163648 |