Anticarsia gemmatalis Nucleopolyhedrovirus from Soybean Crops in Tamaulipas, Mexico: Diversity and Insecticidal Characteristics of Individual Variants and their Co-Occluded Mixtures
[EN] In 1999, Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) was introduced into a major soybean-growing region in Tamaulipas, Mexico, for control of its lepidopteran host, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae). The virus introduction proved to be highly successful in controlling this agronomically important pest. In order to determine the genotypic diversity and insecticidal traits of Mexican AgMNPVs, we obtained 30 field-collected isolates from Tamaulipas State. Five distinct variants (genotypes 1–5) were identified from plaques replicated in A. gemmatalis larvae by examination of restriction profiles using HindIII. Initial screening indicated that none of the variants, or co-occluded mixtures of variants in different proportions, was more pathogenic than the 30 field isolates mixture or a reference variant from Brazil (AgMNPV-2D). Mean occlusion body production also was similar among genotype variants, the mixture of 30 field isolates and AgMNPV-2D treatments, but was significantly reduced in 1 co-occluded mixture. Speed of kill also was similar among variants (except genotype 1) and their mixtures. Lethal concentration metrics indicated that these results were unlikely due to selection of variants with reduced pathogenicity during the plaque purification process. We conclude that the mixture of 30 field isolates most likely would prove suitable for use as a biological insecticide in the soybean-growing region of Mexico.
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Florida Entomological Society
2018-09-01
|
| Subjects: | Baculovirus, Genotypic mixtures, Pathogenicity, Speed-of-kill, Virus production, Mezclas genotípicas, Patogenicidad, Tiempo de matar, Producción viral, |
| Online Access: | http://hdl.handle.net/10261/191551 http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/501100011109 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|