Architecture of the ESCPE-1 membrane coat

Recycling of membrane proteins enables the reuse of receptors, ion channels and transporters. A key component of the recycling machinery is the endosomal sorting complex for promoting exit 1 (ESCPE-1), which rescues transmembrane proteins from the endolysosomal pathway for transport to the trans-Golgi network and the plasma membrane. This rescue entails the formation of recycling tubules through ESCPE-1 recruitment, cargo capture, coat assembly and membrane sculpting by mechanisms that remain largely unknown. Herein, we show that ESCPE-1 has a single-layer coat organization and suggest how synergistic interactions between ESCPE-1 protomers, phosphoinositides and cargo molecules result in a global arrangement of amphipathic helices to drive tubule formation. Our results thus define a key process of tubule-based endosomal sorting.

Bibliographic Details

Main Authors:	López-Robles, Carlos, Scaramuzza, Stefano, Astorga-Simón, Elsa N., Ishida, Morié, Williamson, Chad D., Baños-Mateos, Soledad, Gil-Cartón, David, Romero-Durana, Miguel, Vidaurrazaga, Ander, Fernández-Recio, Juan, Rojas, Adriana L., Bonifacino, Juan S., Castaño-Díez, Daniel, Hierro, Aitor
Other Authors:	Agencia Estatal de Investigación (España)
Format:	artículo biblioteca
Published:	Nature Publishing Group 2023-07
Online Access:	http://hdl.handle.net/10261/336995
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