Phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity by a new in vitro assay method in berry fruits
An HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3β-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method considered kinetic studies to establish the incubation time to perform the assay. The method here described proved to be an interesting approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other exiting methods. The enzyme activity method developed was applied to strawberry, raspberry, blackberry, redcurrant and blackcurrant fruits. © 2013 Elsevier Ltd. All rights reserved.
| Main Authors: | , , , |
|---|---|
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2014
|
| Subjects: | Flavanone 3β-hydroxylase, Flavonol synthase, Enzyme activity, Berry, Phenylalanine ammonia-lyase, |
| Online Access: | http://hdl.handle.net/10261/91839 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|