Determination of nine carotenoids, retinol, retinyl palmitate and α-tocopherol in control human serum using two internal standards

In the determination of the carotenoids in 39 samples of human serum from Spanish control subjects, those most abundant were found to be β-cryptoxanthin, lycopene, lutein and β-carotene; the least abundant carotenoids were zeaxanthin and α-carotene. Lutein and zeaxanthin, when analysed separately, were present at a ratio of about 3:1 in all the sera studied. One xanthophyll, identified as canthaxanthin, was detected in all serum samples. Other carotenoids, such as γ-carotene and β-apo-8′-carotenal, were found in only some of the samples. Retinol and α-tocopherol were determined simultaneously, as was the tocopherol/cholesterol ratio. Retinyl palmitate was measured in only one of the control subjects, although its elution produced no interference with β-carotene or cis-β-carotene. As internal standards, retinyl acetate was used for retinol and tocopherol acetate for α-tocopherol and the carotenoids.

Bibliographic Details

Main Authors:	Olmedilla Alonso, Begoña, Granado Lorencio, Fernando, Blanco, Inmaculada, Rojas-Hidalgo, E.
Other Authors:	Instituto de Salud Carlos III
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 1992
Online Access:	http://hdl.handle.net/10261/241359 http://dx.doi.org/10.13039/501100004587
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