Early evolution of the T-box transcription factor family
Developmental transcription factors are key players in animal multicellularity, being members of the T-box family that are among the most important. Until recently, T-box transcription factors were thought to be exclusively present in metazoans. Here, we report the presence of T-box genes in several nonmetazoan lineages, including ichthyosporeans, filastereans, and fungi. Our data confirm that Brachyury is the most ancient member of the T-box family and establish that the T-box family diversified at the onset of Metazoa. Moreover, we demonstrate functional conservation of a homolog of Brachyury of the protist Capsaspora owczarzaki in Xenopus laevis. By comparing the molecular phenotype of C. owczarzaki Brachyury with that of homologs of early branching metazoans, we define a clear difference between unicellular holozoan and metazoan Brachyury homologs, suggesting that the specificity of Brachyury emerged at the origin of Metazoa. Experimental determination of the binding preferences of the C. owczarzaki Brachyury results in a similar motif to that of metazoan Brachyury and other T-box classes. This finding suggests that functional specificity between different T-box classes is likely achieved by interaction with alternative cofactors, as opposed to differences in binding specificity.
Main Authors: | , , , , |
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Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
National Academy of Sciences (U.S.)
2013
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Subjects: | Origin multicellularity, Premetazoan evolution, Subfunctionalization, Porifera, Holozoa, |
Online Access: | http://hdl.handle.net/10261/129168 http://dx.doi.org/10.13039/501100003741 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100000781 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000024 http://dx.doi.org/10.13039/100007631 http://dx.doi.org/10.13039/501100011011 |
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Internet
http://hdl.handle.net/10261/129168http://dx.doi.org/10.13039/501100003741
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100000781
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000024
http://dx.doi.org/10.13039/100007631
http://dx.doi.org/10.13039/501100011011