Search for proteomic biomarkers related to bovine pre-slaughter stress using liquid isoelectric focusing (OFFGEL) and mass spectrometry

Proteome changes derived from animals that have suffered pre-slaughter stress are a fact. In this study, Proteomic analysis was carried out on 20 bovine loin samples from Asturiana de los Valles and crossbreds cattle previously classified as normal and DFD meat at 24 h post-mortem using pH measurements. Sarcoplasmic sub-proteome of Longissimus thoracis at 24 h post-mortem was fractionated by the use of liquid isoelectric focusing (OFFGEL) in the pH range 3–10, followed by SDS-PAGE analysis of each retrieved fraction. The protein fractionation profile showed high reproducibility along the different sample groups. Five protein bands showed significant differences (p < 0.05) between the two groups, allowing discrimination between them. Proteins present in these bands, which were identified by LC-MS, were actin, phosphoglucomutase-1, alpha-crystallin B, heat shock protein beta-6 and heat shock protein beta-1.

Bibliographic Details

Main Authors:	Fuente García, Claudia, Aldai, Noelia, Sentandreu, Enrique, Oliván, Mamen, García-Torres, Susana, Franco, Daniel, Zapata, Carlos, Sentandreu, Miguel Angel
Other Authors:	CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2018-10-29
Subjects:	DFD meat, Pre-slaughter stress, OFFGEL, Protein biomarkers, Mass spectrometry,
Online Access:	http://hdl.handle.net/10261/206641 http://dx.doi.org/10.13039/100007652
Tags:	Add Tag No Tags, Be the first to tag this record!