First evidence of a retrotransposon-like element in olive (Olea europaea): implications in plant variety identification by SCAR-marker development

When developing SCARs by sequencing RAPD markers useful for olive variety identification, one RAPD sequence of 407 bp has been identified that shows significant DNA homology with more than 160 retrotransposon-like sequences. A generally coherent phylogenetic tree has been constructed based on the homologous retrotransposon-like sequences, reflecting genetic distances in 35 species belonging to eight kingdoms. The implications of this finding in the development of SCAR markers are discussed. In addition, specific oligonucleotide primers have been developed to amplify the DNA region and have a practical application as an internal amplification control in SCAR-based multiplexed PCRs. To our knowledge, this is the first report of a retrotransposon-like element in olive.

Bibliographic Details

Main Authors:	Hernández Molina, Pilar, Rosa, Raúl de la, Rallo, Luis, Martín, Antonio, Dorado, Gabriel
Other Authors:	Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Format:	artículo biblioteca
Language:	English
Published:	Springer Nature 2001-05
Subjects:	Kingdom and biological evolution, Phylogenetic tree, RAPD-PCR, Retrotransposon-like sequence, SCAR,
Online Access:	http://hdl.handle.net/10261/184284 http://dx.doi.org/10.13039/501100007273
Tags:	Add Tag No Tags, Be the first to tag this record!