The Transcriptome of Verticillium dahliae Responds Differentially Depending on the Disease Susceptibility Level of the Olive (Olea europaea L.) Cultivar

Among biotic constraints affecting olive trees cultivation worldwide, the soil-borne fungus <i>Verticillium dahliae</i> is considered one of the most serious threats. Olive cultivars display differential susceptibility to the disease, but our knowledge on the pathogen’s responses when infecting varieties differing in susceptibility is scarce. A comparative transcriptomic analysis (RNA-seq) was conducted in olive cultivars Picual (susceptible) and Frantoio (tolerant). RNA samples originated from roots during the first two weeks after inoculation with <i>V. dahliae</i> defoliating (D) pathotype. <i>Verticillium dahliae</i> mRNA amount was overwhelmingly higher in roots of the susceptible cultivar, indicating that proliferation of pathogen biomass is favored in ‘Picual’. A significant larger number of <i>V. dahliae</i> unigenes (11 fold) were only induced in this cultivar. Seven clusters of differentially expressed genes (DEG) were identified according to time-course expression patterns. Unigenes potentially coding for niche-adaptation, pathogenicity, virulence and microsclerotia development were induced in ‘Picual’, while in ‘Frantoio’ expression remained negligible or null. <i>Verticillium dahliae</i> D pathotype transcriptome responses are qualitatively and quantitatively different, and depend on cultivar susceptibility level. The much larger <i>V. dahliae</i> biomass found in ‘Picual’ roots is a consequence of both host and pathogen DEG explaining, to a large extent, the higher aggressiveness exerted over this cultivar.

Bibliographic Details

Main Authors:	Jiménez-Ruiz, Jaime, Leyva Pérez, M. de la O, Gómez-Lama Cabanás, Carmen, Barroso-Albarracín, Juan Bautista, Luque, Francisco, Mercado-Blanco, Jesús
Other Authors:	Consejo Superior de Investigaciones Científicas (España)
Format:	artículo biblioteca
Published:	Multidisciplinary Digital Publishing Institute 2019-03-27
Subjects:	Defoliating pathotype, Effector, Pathogenicity, RNA-seq, Susceptibility, Vascular pathogen, Verticillium dahliae transcriptome, Verticillium wilt of olive,
Online Access:	http://hdl.handle.net/10261/180707 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100011011
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