An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine
In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis ofPtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae)fps1 − mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin.
| Autores principales: | , , , , |
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| Formato: | article biblioteca |
| Idioma: | eng |
| Materias: | F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale, Pinus taeda, Embryogénèse somatique, expression des gènes, Transcription inverse, Xenopus laevis, Saccharomyces cerevisiae, http://aims.fao.org/aos/agrovoc/c_5913, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_35129, http://aims.fao.org/aos/agrovoc/c_49063, http://aims.fao.org/aos/agrovoc/c_6724, |
| Acceso en línea: | http://agritrop.cirad.fr/597495/ http://agritrop.cirad.fr/597495/1/Ciavatta%20et%20al%2C%202001.pdf |
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