The nitrate transporter family protein LjNPF8.6 controls the N-fixing nodule activity
N-fixing nodules are new organs formed on legume roots as a result of the beneficial interaction with soil bacteria, rhizobia. The nodule functioning is still a poorly characterized step of the symbiotic interaction, as only a few of the genes induced in N-fixing nodules have been functionally characterized. We present here the characterization of a member of the Lotus japonicus nitrate transporter1/peptide transporter family, LjNPF8.6. The phenotypic characterization carried out in independent L. japonicus LORE1 insertion lines indicates a positive role of LjNPF8.6 on nodule functioning, as knockout mutants display N-fixation deficiency (25%) and increased nodular superoxide content. The partially compromised nodule functioning induces two striking phenotypes: anthocyanin accumulation already displayed 4 weeks after inoculation and shoot biomass deficiency, which is detected by long-term phenotyping. LjNPF8.6 achieves nitrate uptake in Xenopus laevis oocytes at both 0.5 and 30 mm external concentrations, and a possible role as a nitrate transporter in the control of N-fixing nodule activity is discussed.
| Main Authors: | , , , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | P34 - Biologie du sol, P35 - Fertilité du sol, Fixation de l'azote, bactérie fixatrice de l'azote, Rhizobium, Lotus, formation de nodosités, gène de fixation de l'azote, Xenopus laevis, nitrate, http://aims.fao.org/aos/agrovoc/c_5196, http://aims.fao.org/aos/agrovoc/c_27939, http://aims.fao.org/aos/agrovoc/c_6563, http://aims.fao.org/aos/agrovoc/c_4442, http://aims.fao.org/aos/agrovoc/c_6643, http://aims.fao.org/aos/agrovoc/c_331014, http://aims.fao.org/aos/agrovoc/c_49063, http://aims.fao.org/aos/agrovoc/c_5187, |
| Online Access: | http://agritrop.cirad.fr/594990/ http://agritrop.cirad.fr/594990/1/Valkov%20et%20al.%2C%202017%20The%20Nitrate%20Transporter%20Family%20Protein%20LjNPF8.6%20Controls%20the%20N-Fixing%20Nodule%20Activity.pdf |
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