Background West Nile virus (WNV) is an emergent pathogen that is widely distributed in North and Central America. The recent introduction in South America has focused attention on the spread of WNV across Southern American countries. The transmission network involves mosquitoes, birds, horses and humans. Methods The serological evaluation of sera from 678 equids and 478 birds was performed using a WNV-specific blocking ELISA, and only the positive results were confirmed by plaque reduction neutralisation tests (PRNTs). Molecular analysis was performed on sera from 992 healthy equids and on 63 macerates of brains from equids that died of encephalitis and had previously tested negative for other pathogens. We also tested swabs from 928 birds. The samples analysed were collected in different biomes of Brazil. Results We identified WNV antibodies by ELISA in thirteen equids and five birds, and PRNT90 confirmed WNV positivity in four equid samples collected in 2009 in an area between the Amazon and the Pantanal. None of the ELISA positive bird samples were confirmed by PRNT90, and all samples tested by RT-PCR were negative. Conclusion WNV circulation is confirmed by this large scale survey even in the absence of detection of clinical cases.
Bibliographic Details
| Main Authors: |
Ometto, Tatiana,
Durigon, Edison Luiz,
De Araujo, Jansen,
Aprelon, Rosalie,
Moura de Aguiar, Daniel,
Cavalcante, Cuacyara Tenorio,
Melo, Rosane Marini,
Levi, José Eduardo,
Mendes de Azevedo Júnio, Severino,
Petry, Maria Virgínia,
Neto, Isaac Simão,
Serafini, Patrícia,
Villalobos, Eliana,
Cunha, Elenice Maria Sequetin,
Lara, Maria do Carmo Custódio S. H.,
Ferreira Dales Nava, Alessandra,
Schiavo Nardi, Marcello,
Hurtado, Renata,
Rodrigues, Roberta B.,
Sherer, Angelo Luís,
Sherer, Janete de Fátima Martins,
Geraldi, Marcelo Plaisant,
Moraes de Seixas, Marina Maria,
Peterka, Cassio,
De Souza Bandeira, Debora,
Pradel, Jennifer,
Vachiery, Nathalie,
Labruna, Marcelo Bahia,
Aranha de Camargo, Luiz Marcelo,
Lanciotti, Robert,
Lefrançois, Thierry |
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| Format: | article
biblioteca
|
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| Language: | eng |
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| Subjects: | L73 - Maladies des animaux,
U30 - Méthodes de recherche,
L72 - Organismes nuisibles des animaux,
fièvre,
flavivirus,
surveillance épidémiologique,
sérologie,
biologie moléculaire,
Equidae,
oiseau,
migration animale,
Enquête pathologique,
technique immunologique,
sérum sanguin,
Test ELISA,
http://aims.fao.org/aos/agrovoc/c_10810,
http://aims.fao.org/aos/agrovoc/c_2963,
http://aims.fao.org/aos/agrovoc/c_16411,
http://aims.fao.org/aos/agrovoc/c_27081,
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http://aims.fao.org/aos/agrovoc/c_32926,
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http://aims.fao.org/aos/agrovoc/c_3807,
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http://aims.fao.org/aos/agrovoc/c_15968,
http://aims.fao.org/aos/agrovoc/c_1070, |
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| Online Access: | http://agritrop.cirad.fr/571933/
http://agritrop.cirad.fr/571933/1/document_571933.pdf
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