The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding

Resistance (R) proteins recognize pathogen avirulence (Avr) proteins by direct or indirect binding and are multidomain proteins generally carrying a nucleotide binding (NB) and a leucine-rich repeat (LRR) domain. Two NB-LRR protein-coding genes from rice (Oryza sativa), RGA4 and RGA5, were found to be required for the recognition of the Magnaporthe oryzae effector AVR1-CO39. RGA4 and RGA5 also mediate recognition of the unrelated M. oryzae effector AVR-Pia, indicating that the corresponding R proteins possess dual recognition specificity. For RGA5, two alternative transcripts, RGA5-A and RGA5- B, were identified. Genetic analysis showed that only RGA5-A confers resistance, while RGA5-B is inactive. Yeast two-hybrid, coimmunoprecipitation, and fluorescence resonance energy transfer-fluorescence lifetime imaging experiments revealed direct binding of AVR-Pia and AVR1-CO39 to RGA5-A, providing evidence for the recognition of multiple Avr proteins by direct binding to a single R protein. Direct binding seems to be required for resistance as an inactive AVR-Pia allele did not bind RGA5-A. A small Avr interaction domain with homology to the Avr recognition domain in the rice R protein Pik-1 was identified in the C terminus of RGA5-A. This reveals a mode of Avr protein recognition through direct binding to a novel, non-LRR interaction domain.

Bibliographic Details

Main Authors:	Cesari, Stella, Thilliez, Gaëtan, Ribot, Cécile, Chalvon, Véronique, Michel, Corinne, Jauneau, Alain, Rivas, Susana, Alaux, Ludovic, Kanzaki, Hiroyuki, Okuyama, Yudai, Morel, Jean-Benoit, Fournier, Elisabeth, Tharreau, Didier, Terauchi, Ryohei, Kroj, Thomas
Format:	article biblioteca
Language:	eng
Subjects:	F30 - Génétique et amélioration des plantes, H20 - Maladies des plantes, Oryza sativa, Magnaporthe, résistance aux maladies, résistance génétique, mécanisme de défense, protéine, phylogénie, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_31827, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_35130, http://aims.fao.org/aos/agrovoc/c_35269, http://aims.fao.org/aos/agrovoc/c_6259, http://aims.fao.org/aos/agrovoc/c_13325,
Online Access:	http://agritrop.cirad.fr/569450/ http://agritrop.cirad.fr/569450/1/document_569450.pdf
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