Enzymatic production of conjugated linoleic acid monoacylglycerols from dehydrated isomerized castor bean oil
The goal of this study was to produce conjugated linoleic acid (CLA) monoacylglycerols (MAGs). A chemicoenzymatic process was employed starting from castor bean oil, which was chemically dehydrated and isomerized to obtain new modified triacylglycerols (TAGs) with a very high proportion of CLA fatty acids (>95%). Such TAGs were then used in enzymatic glycerolysis reaction using Candida antarctica B lipase at 55C in tert-butyl alcohol as a solvent. The effect of enzyme load and substrate molar ratio on the production yield of MAGs was evaluated and optimized using response surface methodology with a two-factor and three-level factorial design. A satisfactory linear correlation existed between experimental results and predicted values (R2 = 0.97 and R2adj. = 0.94). Using the contour plot generated from the model for MAG production yield, optimum parameters could be obtained corresponding to an 11/1 glycerol/TAG molar ratio and an enzyme load of 2% (% w/w of both substrates).
| Main Authors: | , , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | Q02 - Traitement et conservation des produits alimentaires, Q60 - Traitement des produits agricoles non alimentaires, huile de ricin, Ricinus communis, acide linoléique, biosynthèse, triacylglycérol lipase, triglycéride, Candida, acylglycérole, activité enzymatique, isomérisation, séchage, http://aims.fao.org/aos/agrovoc/c_25466, http://aims.fao.org/aos/agrovoc/c_6600, http://aims.fao.org/aos/agrovoc/c_4352, http://aims.fao.org/aos/agrovoc/c_928, http://aims.fao.org/aos/agrovoc/c_24095, http://aims.fao.org/aos/agrovoc/c_14992, http://aims.fao.org/aos/agrovoc/c_1247, http://aims.fao.org/aos/agrovoc/c_3297, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_27561, http://aims.fao.org/aos/agrovoc/c_2402, |
| Online Access: | http://agritrop.cirad.fr/543238/ http://agritrop.cirad.fr/543238/1/document_543238.pdf |
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