Evaluation of the mitochondrial COI gene as a useful genetic marker for Myndus crudus and other Myndus spp.
In the Americas and Caribbean, the lethal yellowing (LY) phytoplasma causes a disease of palms transmitted by the leafhopper Myndus crudus (family, Cixiidae), which may not be the sole vector. In Vanuatu and Ghana LY-like diseases also are rampant in coconut palm but M. taffini is thought to be a vector. Recently, high mortality in disease-resistant palms has occurred widely. The availability of a molecular marker would make possible the tracking of distribution and dispersal behaviors of Myndus species in relation to epidemic and non-epidemic areas. We employed PCR and mtCOI primers [F-C1-J-2195, R-L2-N-3014] to amplify from DNA extracts a 780 bp fragment. Phylogenetic analysis sorted putative Myndus spp. into three main clades: adiopodoumeensis (Ghana), crudus (Caribbean), and taffinii (Vanuvatu), the latter which comprised two sister groups. A fourth Myndus spp. from Oaxaca MEX grouped uniquely from other New World collections and herein has been identified as a new, putative vector species. (Texte intégral)
| Main Authors: | , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | H20 - Maladies des plantes, U30 - Méthodes de recherche, Myndus, Myndus crudus, Myndus taffini, marqueur génétique, vecteur de maladie, phylogénie, identification, séquence nucléotidique, PCR, génétique mitochondriale, http://aims.fao.org/aos/agrovoc/c_27267, http://aims.fao.org/aos/agrovoc/c_27268, http://aims.fao.org/aos/agrovoc/c_27269, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_8164, http://aims.fao.org/aos/agrovoc/c_13325, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_27859, http://aims.fao.org/aos/agrovoc/c_5159, http://aims.fao.org/aos/agrovoc/c_3253, http://aims.fao.org/aos/agrovoc/c_7608, |
| Online Access: | http://agritrop.cirad.fr/542457/ |
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