Analise da expressão de genes candidatos para a tolerância a seca em folhas de clones de Coffea canephora Var. Cornillon, caracterizados fisiologiamente

Candidate genes involved in coffee drought tolerance were selected by in silico analysis (Electronic Northern) of SH2 and SH3 cDNA libraries of the Coffee Genome database. Eighteen "contigs" with in silico differential expression were identified and characterized by Northern-blot experiments using total RNA extracted from leaves of drought tolerant and susceptible clones of Coffea canephora var. Conillon grown in greenhouse under controlled conditions. Physiological analyses showed that the clone 22 (drought susceptible) presented faster decrease of leaf water potential than resistant ones that was attributed to its higher stomatal conductance. Below -3.0MPa, this clone also showed lower photosynthetic activity correlated with elevated oxidative stress. For most of the candidate genes selected by "Electronic Northern" approach, differential gene expression was confirmed by conventional Northern-blot analyses. Interestingly, some of them also presented differential expression patterns between the four clones tested (drought susceptible vs. drought tolerant). Research perspectives are discussed based on these results.

Bibliographic Details

Main Authors:	Marraccini, Pierre, Da Silva, Vânia A., Elbelt, Sonia, Guimarães, Breno L.S., Loureiro, Marcelo E., Damatta, Fabio M., Ferrão, Maria Amélia G., Da Fonseca, Aymbiré F.A., Da Silva, Felipe, Andrade, Alan Carvalho
Format:	conference_item biblioteca
Language:	por
Published:	s.n.
Subjects:	F30 - Génétique et amélioration des plantes, H50 - Troubles divers des plantes,
Online Access:	http://agritrop.cirad.fr/540487/ http://agritrop.cirad.fr/540487/1/document_540487.pdf
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