Soluble sugars, enzymatic activities and gene expression during development of coffee fruits submitted to shade condition

Effects of shade (50% light) condition on development and sugar metabolism was analyzed in fruits of Coffea arabica L. var. IAPAR 59. Fresh weight measurements of separated tissues (pericarp, perisperm and endosperm) showed that the increased size observed in beans of shaded plants was correlated with high perisperm development. Reducing sugar contents in the pericarp and endosperm of fruits from shaded plants appeared higher than in the same tissues of control ("full-sun") plants. On the contrary, sucrose content was slightly lower in shade than in control beans. At the enzymatic level, sucrose synthase activities detected in the latest stages (197-231 DAF) of perisperm development from shaded plants were higher than from of control, confirming the importance of this tissue in coffee fruit development. At the molecular level, the CaSUS1 and CaSUS2 gene expression was also analyzed in the endosperm from control and shaded fruits. Expression level of CaSUS2 was higher in mature beans (260 DAF) of shaded plants than in control plants (231 DAF). These results showed that light condition affect sucrose metabolism of coffee beans.

Bibliographic Details

Main Authors:	Marraccini, Pierre, Geromel, Clara, Ferreira, Lucia Pires, Perreira, Luiz Filipe Protasio, Leroy, Thierry, Pot, David, Davrieux, Fabrice, Ribeyre, Fabienne, Guyot, Bernard, Scholz, Maria Brigida dos Santos, Androcioli Filho, Armando, Vieira, Luiz Gonzaga Esteves, Mazzafera, Paulo
Format:	conference_item biblioteca
Language:	eng
Published:	ASIC
Subjects:	F30 - Génétique et amélioration des plantes, F62 - Physiologie végétale - Croissance et développement, F60 - Physiologie et biochimie végétale, Coffea arabica, métabolisme des glucides, développement biologique, fruit (botanique), teneur en glucides, ombrage, ligase, activité enzymatique, saccharose, http://aims.fao.org/aos/agrovoc/c_1721, http://aims.fao.org/aos/agrovoc/c_1299, http://aims.fao.org/aos/agrovoc/c_921, http://aims.fao.org/aos/agrovoc/c_3119, http://aims.fao.org/aos/agrovoc/c_1298, http://aims.fao.org/aos/agrovoc/c_7020, http://aims.fao.org/aos/agrovoc/c_4320, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_7495,
Online Access:	http://agritrop.cirad.fr/540056/ http://agritrop.cirad.fr/540056/1/ID540056.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!