Co-inoculation of Acacia mangium with Glomus intraradices and Bradyrhizobium sp. in aeroponic culture

Acacia mangium grown in aeroponic culture was co-inoculated with selected strains of Bradyrhizobium sp. and Glomus intraradices. A single-step technique using alginate as an embedding and sticking agent for an inoculum composed of arbuscular mycorrhiza (AM)-infected sheared roots was used to infect plants. This method resulted in the successful establishment of AM in 100% of the inoculated plants after 7 weeks. The results indicated that dual microbial inoculation with Glomus intraradices strain S-043 and Bradyrhizobium strain AUST 13C stimulated the growth of A. mangium in aeroponic culture. The effects of single and dual microbial inoculations were also evaluated at two levels of P in the nutrient medium. A concentration of 5 mg P kg-1 stimulated the development of AM without affecting plant development or establishment of Bradyrhizobium symbiosis. In contrast, saplings supplemented with a higher concentration of P (25 mg kg-1) alone or co-inoculated with Bradyrhizobium had lower AM frequencies.

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Bibliographic Details
Main Authors: Weber, Jean, Ducousso, Marc, Tham, Foong Yee, Nourissier-Mountou, Sophie, Galiana, Antoine, Prin, Yves, Lee, Sing Kong
Format: article biblioteca
Language:eng
Subjects:F62 - Physiologie végétale - Croissance et développement, inoculation, Glomus intraradices, Bradyrhizobium, Fixation de l'azote, substance de croissance végétale, http://aims.fao.org/aos/agrovoc/c_3879, http://aims.fao.org/aos/agrovoc/c_35499, http://aims.fao.org/aos/agrovoc/c_27138, http://aims.fao.org/aos/agrovoc/c_5196, http://aims.fao.org/aos/agrovoc/c_5968,
Online Access:http://agritrop.cirad.fr/527094/
http://agritrop.cirad.fr/527094/1/527094.pdf
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