Nonradioactive sequence-tagged microsatellite site analyses : a method transferable to the tropics
Utilization of existing isozyme analysis facilities to detect sequence-tagged microsatellite site (STMS) polymorphism or any simple sequence repeat (SSR) variation is described. Different parameters concerning the difficulties in transferring molecular techniques to less sophisticated laboratory infrastructures (i.e. tropical outstations) are discussed (e.g. reproducibility, efficacy, precision). Nonradioactive STMS analysis is bound to foster collaborative research between "biodiversity" and "biotechnology" centers.
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| Main Authors: | , , , , , |
|---|---|
| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F30 - Génétique et amélioration des plantes, Musaceae, microsatellite, marqueur génétique, variation génétique, carte génétique, transfert de technologie, technique analytique, isoenzyme, http://aims.fao.org/aos/agrovoc/c_4998, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_24002, http://aims.fao.org/aos/agrovoc/c_7645, http://aims.fao.org/aos/agrovoc/c_1513, http://aims.fao.org/aos/agrovoc/c_15987, |
| Online Access: | http://agritrop.cirad.fr/390266/ http://agritrop.cirad.fr/390266/1/390266.pdf |
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