Molecular mapping of cocoa chromosomes
A map of cocoa genome has been constructed using isozymes, RAPD, c-DNA and genomic probes. c-DNA library were constructed from germinated seeds of an Amelonado clone.The selection of probes was made on 5 genotypes, using 5 restriction enzymes. 500 tested probes, 33 % of c-DNA and 44 % of genomic revealed polymorphic pattrns. The segregations of markers were studied on a "back-cross type"'mapping population of 100 individuals. After the analises were performed using Mapmaker V.2.0 with a lod score = 3 and 0 = 0.35, it was possible to construct map integrating close to 170 RFLP, RAPD and isozymes markers.
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| Main Authors: | , , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
CIRAD
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| Subjects: | F30 - Génétique et amélioration des plantes, Theobroma, marqueur génétique, RAPD, RFLP, carte génétique, http://aims.fao.org/aos/agrovoc/c_7712, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_36341, http://aims.fao.org/aos/agrovoc/c_34255, http://aims.fao.org/aos/agrovoc/c_24002, |
| Online Access: | http://agritrop.cirad.fr/389740/ |
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