Reliable improved molecular detection of coconut lethal yellowing phytoplasma and reduction of associated disease through field management strategies
The relentless spread of the fatal disease lethal yellowing throughout the coconut growing areas is having a serious economic impact on many vulnerable communities, and therefore effective management is required. Phytoplasmas from the 16SrIV group are the associated agents. Improved detection methods for this phytoplasma DNA were developed including: a multiplex direct-PCR system, PCR on 16S rRNA and hemolysin genes; a SybrGreen system based on the GroEL gene; and real-time PCR using TaqMan probes based on the 16S rDNA and GroEL gene. With these methods increased sensitivity and specificity for the detection of this phytoplasma together with quantization capability. These improved methods are part of a package for the integrated management of lethal yellowing spread that is currently tested in coconut farms in Jamaica and show that diseased incidence can be reduced.
| Main Authors: | , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article biblioteca |
| Language: | eng |
| Subjects: | info:eu-repo/classification/Autores/DISEASE INCIDENCE, info:eu-repo/classification/Autores/LETHAL YELLOWING, info:eu-repo/classification/Autores/MANAGEMENT, info:eu-repo/classification/Autores/PHYTOPLASMAS, info:eu-repo/classification/cti/2, |
| Online Access: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/763 |
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