Carrageenan-fed and control microbiota DataSet

[Description of methods used for collection/generation of data] The three colon reactors (R1, R2 and R3) of the BFBL gut simulator were inoculated (1%) with child (5-7 years old) pooled fecal sample. After the microbiota stabilization stage, carrageenan was added to the system at increasing concentrations (0.3 g/L, 1 g/L, 2 g/L and 3 g/L), three times per day for 7 days for each concentration (samples CGN). In addition, another run without addition of carrageenan to the BFBL gut simulator was carried out for the same period of time to assess changes due to the evolution of the bacterial populations over time under these experimental conditions (samples C). During the experimental set up, samples were collected every 24 h from the three colon reactors and centrifuged (11,200 ×g during 10 min at 4 °C), and the pellet and supernatant were stored separately. Bacterial genomic DNA from the pellet was extracted according to the manufacturer’s instruction of the EZNA® Bacterial DNA Kit (Omega Biotek) and using a FastPrep equipment for mechanical lysis (Bio 101 FastPrep FP120, Savant Instruments). DNA samples were sent to Novogene Europe (Cambridge, UK) for sequencing the V3-V4 region of the 16S rRNA gene amplified by using 341F (5′- CCTAYGGGRBGCASCAG-3′) and 806R (5′-GGA CTACNNGGGTATCTAAT-3′) primers. The PCR products were sequenced on a paired-end Illumina platform (NovaSeq 6000, Illumina) to generate 250 bp paired-end raw reads.

Bibliographic Details

Main Authors:	Bellanco-Sevilla, Alicia, Requena, Teresa, Martínez-Cuesta, M. Carmen
Other Authors:	Ministerio de Ciencia e Innovación (España)
Format:	dataset biblioteca
Language:	English
Published:	DIGITAL.CSIC 2021
Subjects:	Metagenomics, Barrier Function, Additives, Carrageenan, Microbiome,
Online Access:	http://hdl.handle.net/10261/351072 https://doi.org/10.20350/digitalCSIC/16165
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