Neuroprotective potential of terpenoid-rich extracts from orange juice by-products obtained by pressurized liquid extraction

Pressurized liquid extraction (PLE) conditions were optimized to improve the recovery of orange (Citrus sinensis) by-products terpenoids. The neuroprotective potential of the PLE extracts were tested against a set of in-vitro assay (antioxidant (ABTS), reactive oxygen/nitrogen species (ROS/RNS)) as well as enzymatic tests (acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and lipoxygenase (LOX)). Gas chromatography coupled to high-resolution mass spectrometry (GC-q-TOF-MS) analysis revealed a higher enrichment in mono- and sesquiterpenoids of the PLE extracts with the highest neuroprotection capacity. In-silico molecular docking analysis showed the specific interaction of representative terpenoids with enzymes active sites. The results demonstrate that the selected extract at 100 °C and 30 minutes possesses high antioxidant (ABTSIC50 = 13.5 μg mL−1; ROSIC50 = 4.4 μg mL−1), anti-cholinesterase (AChEIC50 = 137.1 vg L−1; BChEIC50 = 147.0 μg mL−1) and anti-inflammatory properties (against IL-6 and LOXIC50 = 76.1 μg mL−1), with low cytotoxicity and protection against L-glutamic acid in cell models.

Bibliographic Details

Main Authors:	Sánchez-Martínez, J. David, Álvarez-Rivera, Gerardo, Gallego, Rocío, Bittencourt Fagundes, Mariane, Valdés, Alberto, Mendiola, J. A., Ibáñez, Elena, Cifuentes, Alejandro
Other Authors:	Ministerio de Educación y Formación Profesional (España)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2022
Subjects:	Terpenoids, Pressurized liquid extraction, Neuroprotective, Orange juice by-products, Anti-inflammatory, Green-extraction, GC-q-TOF-MS,
Online Access:	http://hdl.handle.net/10261/285144 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003329
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