System-wide molecular dynamics of endothelial dysfunction in Gram-negative sepsis
[Background]: Inflammation affecting whole organism vascular networks plays a central role in the progression and establishment of several human diseases, including Gram-negative sepsis. Although the molecular mechanisms that control inflammation of specific vascular beds have been partially defined, knowledge lacks on the impact of these on the molecular dynamics of whole organism vascular beds. In this study, we have generated an in vivo model by coupling administration of lipopolysaccharide with stable isotope labeling in mammals to mimic vascular beds inflammation in Gram-negative sepsis and to evaluate its effects on the proteome molecular dynamics. Proteome molecular dynamics of individual vascular layers (glycocalyx (GC), endothelial cells (EC), and smooth muscle cells (SMC)) were then evaluated by coupling differential systemic decellularization in vivo with unbiased systems biology proteomics.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
BioMed Central
2020
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| Online Access: | http://hdl.handle.net/10261/228724 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100001459 http://dx.doi.org/10.13039/501100001381 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 |
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