Short communication: Production of antihypertensive peptide HLPLP by enzymatic hydrolysis: Optimization by response surface methodology
This study evaluates the potential ability of proteolytic enzymes to release the antihypertensive peptide HLPLP, β-casein f(134-138), from caseinate. Corolase PP (Röhm GmbH & Co. KG, Darmstadt, Germany) was found as the most appropriate enzyme to produce this peptide. The optimization of the main experimental variables involved in the process [concentration of Corolase PP, concentration of Peptidase 433P (Biocatalysts Ltd., Parc Nantgarw, UK), and the hydrolysis time on the HLPLP concentration, expressed as area of peak] were studied using a central composite face design. The optimum conditions to obtain the maximum concentration of HLPLP provided by the statistical program were a concentration of Corolase PP of 60. mg/g of protein and hydrolysis time of 24. h. The use of the Peptidase 433P did not increase the amount of the active peptide. The obtained hydrolysate might be used as functional ingredient with antihypertensive properties. © 2012 American Dairy Science Association.
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
Elsevier
2012
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| Subjects: | Antihypertensive peptides, Enzymatic hydrolysis, Caseinate, Response surface methodology, |
| Online Access: | http://hdl.handle.net/10261/101442 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 |
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