Screening of edible mushrooms and extraction by pressurized water (PWE) of 3-hydroxy-3-methyl-glutaryl CoA reductase inhibitors

The methanol/water and particularly the water extracts obtained from 26 mushroom species were able to inhibit the 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGCR) activity to different extent (10-76%). Cultivated mushrooms such as Pleurotus sp. and Lentinula edodes were among the strains which showed higher HMGCR inhibitory capacities. Their inhibitory properties were not largely influenced by cultivation parameters, mushroom developmental stage or flush number. The HMGCR inhibitory activity of L. edodes was concentrated in the cap excluding the gills while in Pleurotus ostreatus it was distributed through all the different tissues. A method to obtain aqueous fractions with high HMGCR inhibitory activity was optimized using an accelerated solvent extractor (ASE) by selecting 10.7. MPa and 25 °C as common extraction conditions and 5 cycles of 5. min each for P. ostreatus fruiting bodies and 15 cycles of 5. min for L. edodes suggesting that the potential HMGCR inhibitors are different in the two selected mushrooms. © 2012 Elsevier Ltd.

Bibliographic Details

Main Authors:	Gil-Ramírez, Alicia, Clavijo, Cristina, Palanisamy, Marimuthu, Ruiz-Rodríguez, Alejandro, Navarro-Rubio, María, Marin, Francisco R., Reglero, Guillermo, Soler-Rivas, Cristina
Other Authors:	European Commission
Format:	artículo biblioteca
Published:	Elsevier 2013
Subjects:	HMGCoA reductase, Pleurotus ostreatus, Lentinus edodes, Accelerated solvent extraction, Cholesterol,
Online Access:	http://hdl.handle.net/10261/100273 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818
Tags:	Add Tag No Tags, Be the first to tag this record!