Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops

Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration, vitrification and droplet-vitrification approaches. They have allowed improvements in survival and recovery after cryopreservation compared with conventional crystallization-based protocols, proving their effectiveness for large scale application with embryos and shoot-tips of different plants. This review addresses the main physical and technological aspects involved in plant cryopreservation methods, illustrating the development of research with three cases: citrus, cassava and potato. These studies demonstrate how cryopreservation strategies are increasingly applied for their successful employment in the genebanks.

Bibliographic Details

Main Authors:	González Amao, M.T., Panta, A., Roca, W., Escobar Pérez, Roosevelt H., Engelmann, F.
Format:	Journal Article biblioteca
Language:	English
Published:	Springer 2007-11-29
Subjects:	cryopreservation, somatic embryogenesis, criopreservación, embriogénesis somática,
Online Access:	https://hdl.handle.net/10568/43329 https://doi.org/10.1007/s11240-007-9303-7
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