Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops
Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vitrification-based procedures such as encapsulation-dehydration, vitrification and droplet-vitrification approaches. They have allowed improvements in survival and recovery after cryopreservation compared with conventional crystallization-based protocols, proving their effectiveness for large scale application with embryos and shoot-tips of different plants. This review addresses the main physical and technological aspects involved in plant cryopreservation methods, illustrating the development of research with three cases: citrus, cassava and potato. These studies demonstrate how cryopreservation strategies are increasingly applied for their successful employment in the genebanks.
| Auteurs principaux: | , , , , |
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| Format: | Journal Article biblioteca |
| Langue: | English |
| Publié: |
Springer
2007-11-29
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| Sujets: | cryopreservation, somatic embryogenesis, criopreservación, embriogénesis somática, |
| Accès en ligne: | https://hdl.handle.net/10568/43329 https://doi.org/10.1007/s11240-007-9303-7 |
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