Linkage mapping of the bovine lysosomal alphamannosidase (MANB) gene
Lysosomal alpha-mannosidase is involved in the metabolism of mannoses in oligosaccharides in lysosomes. Deficiency of alpha-mannosidase, alpha-mannosidosis, has been described as an autosomal recessive storage disease in angus cattle, Galloway cattle and in cats. It is caused by non-synonymous substitutions in the encoding gene (MANB). Primers for MANB were designed from a bovine sequence (Gen Bank accession number U97686) and amplified a 316-bp fragment comprising intron 2 and adjacent exonic sequences. This paper discusses PCR primers, PCR conditions and polymorphism detection; and allele frequencies, inheritance and chromosomal location.
Main Authors: | , , , |
---|---|
Format: | Journal Article biblioteca |
Language: | English |
Published: |
Wiley
1999-06
|
Subjects: | bovidae, genes, genetic maps, lysozyme, |
Online Access: | https://hdl.handle.net/10568/30064 https://doi.org/10.1046/j.1365-2052.1999.00404-3.x |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|