Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus
The size and repetitive nature of the Rhipicephalus microplus genome makes obtaining a full genome sequence fiscally and technically problematic. To selectively obtain gene-enriched regions of this tick's genome, Cot filtration was performed, and Cot-filtered DNA was sequenced via 454 FLX pyrosequencing.
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Main Authors: | , , , , , , , |
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Format: | Journal Article biblioteca |
Language: | English |
Published: |
Springer
2010
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Subjects: | cattle, genetics, |
Online Access: | https://hdl.handle.net/10568/2323 https://doi.org/10.1186/1471-2164-11-374 |
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