Impact of orange essential oil on enteric methane emissions of heifers fed bermudagrass hay

In this study, the effects of orange essential oil (OEO) on the rumen fermentation, nutrient utilization, and methane (CH₄) emissions of beef heifers fed a diet of bermudagrass (Cynodon dactylon) were examined. In addition, in vitro and in situ experiments were conducted. The in vitro experiment consisted of three treatments: control (CTL, no OEO), OEO1 (0.25% OEO), and OEO2 (0.5% OEO). The forage to concentrate ratio was 70:30 (dry matter [DM] basis) in all treatments. No changes in pH, proportions of volatile fatty acids, and the acetate:propionate ratio were observed (P > 0.05). The addition of 0.25% OEO resulted in a reduction in CH₄ production (mL/g) relative to the control (P < 0.05). In the in situ experiment, 5 g of total mixed ration (CTL, OEO1, and OEO2) were incubated for 6, 12, 24, 48, and 72 h. Potential and effective degradability were not affected by OEO supplementation (P > 0.05). In the in vivo study, six crossbred beef heifers (Bos indicus × Bos taurus), fitted with rumen cannulas, were assigned to three different treatments: no additive (CTL), 0.25% OEO (OEO1), and 0.5% OEO (OEO2) in a replicated 3 × 3 Latin square (21-day periods). Heifers were fed at 2.8% body weight. In vivo CH₄ production was measured in open-circuit respiration chambers. Reductions in gross energy consumption, apparent total tract digestibility, and rumen valerate concentration were observed for OEO2 compared to the control (P < 0.05). Additionally, decreases in CH₄ emissions (g/day; P < 0.05) and CH4 (MJ gross energy intake/day; P < 0.05) were observed in response to supplementation of 0.5% OEO as compared to the CTL treatment. Thus, supplementation of 0.5% OEO reduced CH₄ emissions (g/day) by 12% without impacting the DM intake of heifers fed bermudagrass hay as a basal ration.

Bibliographic Details

Main Authors:	Jiménez Ocampo, Rafael, Montoya Flores, María D., Pamanes-Carrasco, Gerardo, Herrera-Torres, Esperanza, Arango, Jacobo, Estarrón-Esponosa, Mirna, Aguilar-Pérez, Carlos F., Araiza-Rosales, Elia E., Guerrero Cervantes, Maribel, Ku-Vera, Juan C.
Format:	Journal Article biblioteca
Language:	English
Published:	Frontiers Media 2022-08-16
Subjects:	cattle, methane, essential oils, feed additives, metabolites, ganado bovino, metano, aceites esenciales, aditivos de piensos,
Online Access:	https://hdl.handle.net/10568/121015 https://doi.org/10.3389/fvets.2022.863910
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