Assessment of the pollution of the bottom sediments in the Azov Sea by biotesting using bioluminescent tests

This study is aimed at the toxicity assessment of the bottom sediments in the Azov Sea. 32 samples of the bottom sediments, taken in Taganrog Bay, as well as in the Northern, Western, Central, Southern, and Eastern regions of the sea, have been investigated. A biotesting method based on whole-cell bacterial lux-biosensors (Vibrio aquamarinus VKPM B-11245, E. coli MG1655 (pRecA-lux), E. coli MG1655 (pKatG-lux), and E. coli MG1655 (pSoxS-lux)) was used to determine the toxicity level. Toxicity analysis of the Azov Sea bottom sediments showed their extensive contamination with pollutants of various nature. Integral toxicity was recorded for the surface sediments of all the investigated stations in the Azov Sea. The pollution level of the bottom sediments was estimated as highly toxic at 31 stations and as toxic at 1 station. The presence of genotoxic substances, both direct and promutagenic, was detected, as well as the presence of substances causing oxidative stress. It is shown that Taganrog Bay, the Central and Eastern regions of the Azov Sea are most exposed to pollution. The most polluted sampling stations for bottom sediments have been identified. Integral toxicity and the toxic effects caused by the presence of genotoxic and prooxidant substances indicate gradual accumulation of pollutants in the bottom sediments of the Azov Sea and its unfavorable environmental state. This study has established the biotesting method involving whole-cell bacterial luminescent sensors as a promising tool for the assessment of the ecotoxicological state of the investigated water body. It is deemed necessary to put further efforts into the identification of toxically active compounds in marine sediments and exploration of their possible sources.

Bibliographic Details

Main Authors:	Al-Rammahi, Amir Aboud, Sazykina, M.A., Barabashin, Timofey, Karchava, Sh.K., Klimova, M.V., Khammami, Margarita, Sazykin, Ivan
Format:	Journal Contribution biblioteca
Language:	Russian
Published:	2022
Subjects:	Lux-биосенсоры, lux-biosensors, Bottom sediments, Vibrio aquamarinus, Toxicity assessment, Whole-cell bacterial luminescent sensors, Интегральная токсичность, Уровень загрязнения, ASFA_2015::B::Bioluminescence, ASFA_2015::S::Sediment pollution, ASFA_2015::B::Biotesting,
Online Access:	http://hdl.handle.net/1834/42525
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