The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride
The purpose of this study was to evaluate the effect of antioxidants, vitamin C and chitosan, on oxidative stress markers in gills of fish during exposure to cadmium chloride. Fish were fed with either of commercial diet (control group), chitosan enriched diet (100 mg Kg-1 feed), Vitamin C enriched diet (100 mg Kg-1 feed), or chitosan + vitamin C enriched diet, and simultaneously exposed to 0.2 mg L-1 cadmium chloride for 21 days. At the end of experiment, oxidative stress biomarkers such as catalase activity, total antioxidant and malondialdehyde as well as cellular biochemical parameters including aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase activities were measured. The aspartate aminotransferase, lactate dehydrogenase and catalase activities as well as malondialdehyde levels significantly increased in gill cells of fish exposed to cadmium chloride, while the cellular total antioxidant levels and alanine aminotransferase activity significantly decreased. Enzyme activities and malondialdehyde levels in fish treated with vitamin C and vitamin C combined with chitosan were returned to the normal level after 21 days. However, administration of vitamin C and chitosan did not have significant effect on the cellular total antioxidant. In conclusion, administration of natural antioxidant such as vitamin C and chitosan may increase the efficiency of the antioxidant defense system and detoxification of gill cells of fish exposed to cadmium chloride.
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Format: | Journal Contribution biblioteca |
Language: | Persian |
Published: |
2016
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Subjects: | Chitosan, Antioxidant defense system, Cyprinus carpio, Common carp, Biomarkers, Vitamin C, Cadmium chloride, Gill, Diet, Feed, Levels, |
Online Access: | http://hdl.handle.net/1834/10833 |
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dig-aquadocs-1834-108332021-05-19T06:45:56Z The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride Mehrepak, M. Banaie, M. Nematdoste Haghi, B. Noori, A. Chitosan Antioxidant defense system Cyprinus carpio Common carp Biomarkers Vitamin C Cadmium chloride Gill Diet Feed Levels The purpose of this study was to evaluate the effect of antioxidants, vitamin C and chitosan, on oxidative stress markers in gills of fish during exposure to cadmium chloride. Fish were fed with either of commercial diet (control group), chitosan enriched diet (100 mg Kg-1 feed), Vitamin C enriched diet (100 mg Kg-1 feed), or chitosan + vitamin C enriched diet, and simultaneously exposed to 0.2 mg L-1 cadmium chloride for 21 days. At the end of experiment, oxidative stress biomarkers such as catalase activity, total antioxidant and malondialdehyde as well as cellular biochemical parameters including aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase activities were measured. The aspartate aminotransferase, lactate dehydrogenase and catalase activities as well as malondialdehyde levels significantly increased in gill cells of fish exposed to cadmium chloride, while the cellular total antioxidant levels and alanine aminotransferase activity significantly decreased. Enzyme activities and malondialdehyde levels in fish treated with vitamin C and vitamin C combined with chitosan were returned to the normal level after 21 days. However, administration of vitamin C and chitosan did not have significant effect on the cellular total antioxidant. In conclusion, administration of natural antioxidant such as vitamin C and chitosan may increase the efficiency of the antioxidant defense system and detoxification of gill cells of fish exposed to cadmium chloride. Published 2017-12-22T12:04:41Z 2017-12-22T12:04:41Z 2016 Journal Contribution Refereed 1026-1354 http://hdl.handle.net/1834/10833 fa pp.31-44 Iran |
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Chitosan Antioxidant defense system Cyprinus carpio Common carp Biomarkers Vitamin C Cadmium chloride Gill Diet Feed Levels Chitosan Antioxidant defense system Cyprinus carpio Common carp Biomarkers Vitamin C Cadmium chloride Gill Diet Feed Levels |
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Chitosan Antioxidant defense system Cyprinus carpio Common carp Biomarkers Vitamin C Cadmium chloride Gill Diet Feed Levels Chitosan Antioxidant defense system Cyprinus carpio Common carp Biomarkers Vitamin C Cadmium chloride Gill Diet Feed Levels Mehrepak, M. Banaie, M. Nematdoste Haghi, B. Noori, A. The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride |
description |
The purpose of this study was to evaluate the effect of antioxidants, vitamin C and chitosan, on
oxidative stress markers in gills of fish during exposure to cadmium chloride. Fish were fed with
either of commercial diet (control group), chitosan enriched diet (100 mg Kg-1 feed), Vitamin C
enriched diet (100 mg Kg-1 feed), or chitosan + vitamin C enriched diet, and simultaneously
exposed to 0.2 mg L-1
cadmium chloride for 21 days. At the end of experiment, oxidative stress
biomarkers such as catalase activity, total antioxidant and malondialdehyde as well as cellular
biochemical parameters including aspartate aminotransferase, alanine aminotransferase and lactate
dehydrogenase activities were measured. The aspartate aminotransferase, lactate dehydrogenase and
catalase activities as well as malondialdehyde levels significantly increased in gill cells of fish
exposed to cadmium chloride, while the cellular total antioxidant levels and alanine
aminotransferase activity significantly decreased. Enzyme activities and malondialdehyde levels in
fish treated with vitamin C and vitamin C combined with chitosan were returned to the normal level
after 21 days. However, administration of vitamin C and chitosan did not have significant effect on
the cellular total antioxidant. In conclusion, administration of natural antioxidant such as vitamin C
and chitosan may increase the efficiency of the antioxidant defense system and detoxification of gill
cells of fish exposed to cadmium chloride. |
format |
Journal Contribution |
topic_facet |
Chitosan Antioxidant defense system Cyprinus carpio Common carp Biomarkers Vitamin C Cadmium chloride Gill Diet Feed Levels |
author |
Mehrepak, M. Banaie, M. Nematdoste Haghi, B. Noori, A. |
author_facet |
Mehrepak, M. Banaie, M. Nematdoste Haghi, B. Noori, A. |
author_sort |
Mehrepak, M. |
title |
The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride |
title_short |
The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride |
title_full |
The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride |
title_fullStr |
The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride |
title_full_unstemmed |
The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride |
title_sort |
protective effect of vitamin c and chitosan on oxidative biomarkers in gills of common carp (cyprinus carpio) exposed to cadmium chloride |
publishDate |
2016 |
url |
http://hdl.handle.net/1834/10833 |
work_keys_str_mv |
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