The protective effect of vitamin C and chitosan on oxidative biomarkers in gills of common carp (Cyprinus carpio) exposed to cadmium chloride
The purpose of this study was to evaluate the effect of antioxidants, vitamin C and chitosan, on oxidative stress markers in gills of fish during exposure to cadmium chloride. Fish were fed with either of commercial diet (control group), chitosan enriched diet (100 mg Kg-1 feed), Vitamin C enriched diet (100 mg Kg-1 feed), or chitosan + vitamin C enriched diet, and simultaneously exposed to 0.2 mg L-1 cadmium chloride for 21 days. At the end of experiment, oxidative stress biomarkers such as catalase activity, total antioxidant and malondialdehyde as well as cellular biochemical parameters including aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase activities were measured. The aspartate aminotransferase, lactate dehydrogenase and catalase activities as well as malondialdehyde levels significantly increased in gill cells of fish exposed to cadmium chloride, while the cellular total antioxidant levels and alanine aminotransferase activity significantly decreased. Enzyme activities and malondialdehyde levels in fish treated with vitamin C and vitamin C combined with chitosan were returned to the normal level after 21 days. However, administration of vitamin C and chitosan did not have significant effect on the cellular total antioxidant. In conclusion, administration of natural antioxidant such as vitamin C and chitosan may increase the efficiency of the antioxidant defense system and detoxification of gill cells of fish exposed to cadmium chloride.
| Main Authors: | , , , |
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| Format: | Journal Contribution biblioteca |
| Language: | Persian |
| Published: |
2016
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| Subjects: | Chitosan, Antioxidant defense system, Cyprinus carpio, Common carp, Biomarkers, Vitamin C, Cadmium chloride, Gill, Diet, Feed, Levels, |
| Online Access: | http://hdl.handle.net/1834/10833 |
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